RNA Interference and Aging

 
RNA interference (RNAi) is finding more and more uses as a post-transcriptional gene-silencer. The introduction of small pieces of double-stranded RNA into an active cell leads to the inactivation of the complementary mRNA strand. It thus is a useful tool to silence genes, as if the mRNA of the gene is sufficient inhibited, the genes protein products won't get made. It's an alternative to creating knock-out animals for experiments, wherein the DNA of a mouse would be genetically engineered so that it lacks a certain gene. The mechanism of mRNA is interesting and complex, as the interfering RNAi associates with endogenous enzyme complexes that subsequently degrade the mRNA.

RNAi has been used to increase lifespan in C. Elegans by inhibiting ubiquinone synthesis, and to create superoxide dismutase deficient drosophila models.

cs-related keywords



Recent Related References:
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  • RNAi silencing of brain klotho potentiates cold-induced elevation of blood pressure via the endothelin pathway. Wang X, Sun Z. Physiol Genomics. 2010 Jan 19. PMID: 20086041
  • Novel modulators of senescence, aging, and longevity: Small non-coding RNAs enter the stage. Grillari J, Grillari-Voglauer R. Exp Gerontol. 2010 Jan 18. PMID: 20080172
  • Aging: dial m for mitochondria. Hur JH, Cho J, Walker DW. Aging (Albany NY). 2010 Jan 26. PMID: 20228940
  • APE1/EXO-3, p53/CEP-1 and thioredoxin are linked in control of aging in C. elegans. Schlotterer A, Hamann A, Kukudov G et al. Aging Cell. 2010 Mar 24. PMID: 20346071
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  • Manipulation of Behavioral Decline in Caenorhabditis elegans with the Rag GTPase raga-1. Schreiber MA, Pierce-Shimomura JT, Chan S et al. PLoS Genet. 2010 May 27. PMID: 20523893
  • Conditional inactivation of MRG15 gene function limits survival during larval and adult stages of Drosophila melanogaster. Zhang H, Li Y, Yang J et al. Exp Gerontol. 2010 Jun 23. PMID: 20600782
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